Generator

Part:BBa_K1075045:Design

Designed by: Marc Schulte   Group: iGEM13_Bonn   (2013-09-23)

pDawn-RBS34-mCherry-TT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3048
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 940
    Illegal NgoMIV site found at 1072
    Illegal NgoMIV site found at 1166
    Illegal NgoMIV site found at 1459
    Illegal NgoMIV site found at 1953
    Illegal NgoMIV site found at 1971
    Illegal NgoMIV site found at 2061
    Illegal AgeI site found at 1291
    Illegal AgeI site found at 2419
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2520
    Illegal BsaI.rc site found at 1402


Design Notes

This part is a combination of the light-inducible BBa_K1075045 and the fluorescent protein mCherry.

mCherry is a fluorescent protein with peak absorption/emission at 587 nm and 610 nm, respectively. It is resistant to photobleaching and is stable. It matures quickly, with a t0.5 of 15 minutes, allowing it to be visualised soon after translation. (Shaner et al. 2004)


Source

mCherry is derived from a protein isolated from Discosoma sp..

References

[http://www.nature.com/nbt/journal/v22/n12/full/nbt1037.html]Shaner, Nathan C; Campbell, Robert E; Steinbach, Paul A; Giepmans, Ben N G; Palmer, Amy E; Tsien, Roger Y (2004). Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. Red fluorescent protein.